Khả năng tiêu hóa và tăng trưởng của cừu được cho ăn cỏ alfalfa khô được xử lý bằng enzyme phân giải xơ và esterase axit ferulic

Thứ tư, 28/10/2015 - 13:41


This study was conducted to determine the effects of fibrolytic enzymes applied at baling, with or without ferulic acid esterase (FAE) producing bacterial inoculant, or to hay at feeding on digestibility and growth performance of lambs.

Prior to starting the animal studies, two runs of replicated 24- and 48-h batch culturein vitro incubations were conducted using control alfalfa hay to select a suitable enzyme and dose. Eleven replicate bales of alfalfa-grass (93.8:6.2) hay (∼500 kg) were produced with the application of one of three treatments: control (water), enzyme applied at baling (Eb; Econase RDE-L, AB Vista, Wiltshire, UK) and enzyme plus ferulic acid esterase producing bacterial additive applied at baling (EIb; 11 GFT, Pioneer HI-Bred Ltd., Chathan, ON, Canada). The mean internal bale temperature after 50 days of storage was greater (P<0.001) for Eb than control and EIb, as was the post-storage hemicellulose concentration (P<0.05). Two animal experiments using lambs were conducted after bales were stored for at least 90 days. The digestibility study was a replicated 4 × 4 Latin square design with 16 lambs and the animal performance study consisted of 32 lambs (8 per treatment) in a randomized complete block design. In both studies lambs received one of four treatments: control, Eb, EIb and enzymes added to control hay at feeding (Ef). In the digestibility study, total tract apparent organic matter (OM) (P=0.07) digestibility tended to be affected by treatment, with OM digestibilities greater for lambs fed Ef compared with lambs fed the other treatments, although differences were small (Ef vs. others; OM, 0.658 vs. 0.646). However, neutral detergent fiber (aNDF) and hemicellulose digestibilities were greatest (P<0.05) for lambs fed Eb, with no differences among the other treatments (aNDF, Eb = 0.480, control = 0.437, Ef = 0.430, EIb = 0.430; hemicellulose, Eb = 0.524, control = 0.460, Ef = 0.458, EIb = 0.446). In both studies there was no effect (P>0.05) of treatment on OM intakes. Average daily gain (ADG, g/d) of lambs in the performance study was greater (P=0.048) for EIb (233) than control (192) and Ef (202), and intermediate for Eb (206). Feed efficiency tended to be affected (P=0.07) by treatment; gain:feed for EIb was 18% greater than control and Eb and Ef were similar to the control. This study showed that applying enzymes to alfalfa hay at baling decreased aerobic stability, and increased fiber content and its digestibility, but ADG and gain:feed of lambs were not improved. Adding FAE producing bacterial inoculant with enzymes at baling improved aerobic stability of hay and ADG and gain:feed of lambs were increased relative to lambs fed control and enzymes applied at feeding. Applying enzymes at feeding increased apparent OM digestibilities but not fiber digestibilities, and had no effect on animal performance. We conclude that fibrolytic enzyme application with FAE producing bacterial inoculant at baling was the most promising method for enhancing performance of lambs fed baled alfalfa hay.


·         ACT = 50 days, accumulated difference in daily temperatures of bales and ambient temperatures within 50 days of storage; 

·         ADF, acid detergent fiber inclusive of residual ash; 

·         ADG, average daily gain; 

·         ADICP,acid detergent insoluble crude protein; 

·         aNDF, neutral detergent fiber inclusive of residual ash; 

·         BW, body weight; 

·         DM, dry matter; 

·         DMD, dry matter degradability; 

·         DMI, dry matter intake; 

·         Eb, fibrolytic enzyme product added at baling; 

·         ENZ1, Econase RDE-L, AB Vista, Wiltshire, UK; 

·         EIb, fibrolytic enzyme plus ferulic acid esterase producing bacterial inoculant added at baling; 

·         Ef, fibrolytic enzyme added at feeding; 

·         FAE, ferulic acid esterase; 

·         FE, feed efficiency; 

·         OM, organic matter; 

·         ENZ2, Rovabio Excel LC, Adisseo, Alpharetta, GA, USA


·         Fibrolytic enzyme; 

·         Fiber; 

·         Alfalfa; 

·         Ferulic acid esterase; 

·         Average daily gain; 

·         Apparent digestibility


Animal Feed Science and Technology

Volume 203, May 2015, Pages 53–66

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